@article {roznik_emergency_2024,
	title = {Emergency myelopoiesis distinguishes multisystem inflammatory syndrome in children from pediatric severe COVID-19},
	journal = {The Journal of Infectious Diseases},
	year = {2024},
	month = {jan},
	pages = {jiae032},
	abstract = {Multisystem inflammatory syndrome in children (MIS-C) is a hyperinflammatory condition caused by recent SARS-CoV-2 infection, but the underlying immunological mechanisms driving this distinct syndrome are unknown.We utilized high dimensional flow cytometry, cell-free (cf) DNA, and cytokine and chemokine profiling to identify mechanisms of critical illness distinguishing MIS-C from severe acute COVID-19 (SAC).Compared to SAC, MIS-C patients demonstrated profound innate immune cell death and features of emergency myelopoiesis (EM), an understudied phenomenon observed in severe inflammation. EM signatures were characterized by fewer mature myeloid cells in the periphery and decreased expression of HLA-DR and CD86 on antigen presenting cells. IL-27, a cytokine known to drive hematopoietic stem cells towards EM, was increased in MIS-C, and correlated with immature cell signatures in MIS-C. Upon recovery, EM signatures decreased, and IL-27 plasma levels returned to normal levels. Despite profound lymphopenia, we report a lack of cfDNA released by adaptive immune cells and increased CCR7 expression on T cells indicative of egress out of peripheral blood.Immune cell signatures of EM combined with elevated innate immune cell-derived cfDNA levels distinguish MIS-C from SAC in children and provide mechanistic insight into dysregulated immunity contributing towards MIS-C, offering potential diagnostic and therapeutic targets.},
	issn = {0022-1899},
	doi = {10.1093/infdis/jiae032},
	url = {https://doi.org/10.1093/infdis/jiae032},
	author = {Roznik, Katerina and Andargie, Temesgen E and Johnston, T Scott and Gordon, Oren and Wang, Yi and Peart Akindele, Nadine and Persaud, Deborah and Antar, Annukka A R and Manabe, Yukari C and Zhou, Weiqiang and Ji, Hongkai and Agbor-Enoh, Sean and Karaba, Andrew H and Thompson, Elizabeth A and Cox, Andrea L}
}
